The silkworm, Bombyx mori L. (Lepidoptera Bombycidae) is an oligophagous insect that mainly feeds on mulberry leaves, the gut of the silkworm, B. mori contains an interesting diversity of microorganisms not usually found elsewhere. These microorganisms produce diversity of enzymes to digest the starch compounds, probably in cooperation with endogenous enzymes. In the present study microorganisms were isolated from the gut of silkworm Bombyx mori and were plated on starch agar plate for amylase activity. A total number of 36 strain were isolated and characterized for amylase activity. Upon screening, one isolate was selected on the basis of maximum hydrolysis for further studies. It was identified based on its phenotypes, biochemical test and 16S rRNA gene sequence as Bacillus megaterium and named strain A.