owever, one of the most serious obstacles to use biodiesel as an alternative fuel is the complicated and costly purification processes involved in its production. Therefore, biodiesel must be purified before being used as a fuel in order to fulfil the EN 14214 and ASTM D6751 standard specifications listed in Table 1; otherwise the methyl esters formed cannot be classified as biodiesel. Removing glycerine from biodiesel is important since the glycerine content is one of the most significant precursors for the biodiesel quality. Biodiesel content of glycerine can be in the form of free glycerine or bound glycerine in the form of glycerides. In this work we refer to the total glycerine, which is the sum of free glycerine and bound glycerine. Severe consequences may result due to the high content of free and total glycerine, such as buildup in fuel tanks, clogged fuel systems, injector fouling and valve deposits.