The proposed biosensor was constructed with LAC immobilized in a new matrix based on Pt NPs in SILP, using MMT clay as the support, and it was successfully applied to the determination of methomyl in carrot and tomato samples using the method of enzymatic inhibition. The high responses obtained using this new material showed that this is a promising field of research, that is, the association of this class of supported catalysts with a biocatalyst for application to the development of more sensitive biosensors