Total viable cells of bacteria and fungi were estimated
using a standard counting method as described previously
(Jung et al., 2014). Doenjang samples (2g) were resuspended
and serially diluted in PBS buffer(137 mM NaCl, 2.7 mM
KCl, 10 mM Na2HPO4, 2mM KH2PO4, and pH7.2).
The diluted supernatants were spread on agar media and
incubated at 30C for 3 days. Respectively,trypticase soy agar
(TSA; BD, USA)and potato dextrose agar (PDA; BD, USA),
each containing 3% (w/v) NaCl, were used for bacterial and
fungal cell counts. Bacterial and fungal cell numbers were
counted as colony forming units (CFU) per g-fresh weigh tof
doenjang.