Decolorization of direct blue 75 by Com.acidovorans- N1 after 24 hours showed detectable differences at dif- ferent inocula sizes. Decolorization using inoculum size
0.5 was optimal, followed by decolorization using in- oculum size 0.2. The lowest result was recorded for the inoculum size of 3 ml. But, decolorization of direct blue
75 by Com. acidovorns-TN1 after 120 hours indicated that the highest color removal was recorded at the 0.2 ml inoculum size. The relationship between decolorization percentage and inoculum size was absent in the deco- lorization of direct blue 75 by Bur. cepace-TN5, where it was found that the best decolorization after 24 hours was a inoculum size of 2 ml, but the best result recorded after
120 hours was for the 1ml inoculum size. The recorded protein content for the decolorization of acid orange 7 & direct blue 75 by Com. acidovorns-TM and Bur. Ce- pace-TN5 had the same behavior, so we are unable to establish a clear relationship between protein content and inoculum size or decolorization percent but in general. The values of protein content of the two strains at any inoculum size with direct blue 75 were higher than those of the two strains at any inoculum size with acid orange
7.