Lignocellulosic biomass such as agricultural and forestry residues and energy crops have been considered as a potential source of biofuels and bioproducts since the latter part of the 20th century. Lignocellulosic biomass such as corn stover usually consist of about 35–45% cellulose enclosed within sheets containing both 20–25% hemicellulose and 15–20% lignin bound to each other through carbon–carbon linkages [5]. Lactic acid bacteria have been traditionally studied and used for conversion of sugars from various sources including biomass [6]. However, both pretreatment and enzymatic hydrolysis will be needed to deconstruct the biomass structure [7]. Traditional lignocellulosic biomass pretreatments are accompanied by addition of chemicals such as mineral acids or base, which will affect the final product separation and purification and add to the operational cost [8]. Such pretreatment processes could further lead to production of sugar degradation products such as furfural and 5-hydroxymethyl furfural (HMF) which are considered as potential fermentation inhibitors for lactic acid producers [9]. The wet explosion pretreatment is a combination of wet oxidation with oxygen and steam explosion and is a method, which has been developed to pretreat any lignocellulosic biomass material demanding no addition of harmful chemicals and resulting in minimal production of sugar degradation products [10]. The wet explosion pretreatment process has been found to increase the accessibility of cellulose to enzymatic action through the oxidative action on lignin and hemicellulose linkages [11] and [12]. The biomass sugars obtained after pretreatment followed by enzymatic hydrolysis are an ideal feedstock for lactic acid bacteria that can consume both C5 (i.e., xylose) and C6 (i.e., glucose) sugars.