conclusions
the human HEK293 cell line was more sensitive to tested fractions with the exception of F1 .
Studies on cytotoxicity of A. sieboldiana flowers on VERO and HEK293 cell lines may prove to be useful in the assessment of non-cytotoxic concentration of fractions that will be used during future research on the biological activity of this plant.
EtOAc fraction F3 showed potent cytotoxic activity, especially on HEK293,and therefore should be further analyzed using appropriate cell lines for potential anticancer properties.