and the condition using 1300 μL was selected for further
experiments.
After optimization of the volume of acetonitrile (1300 μL),
the volume of tetrachloroethylene was evaluated from 20 and
160 μL. Results are shown in Figure 4, and a significant
difference (Tukey−Kramer test) was not observed among 40
and 160 μL of CCl4. Considering the use of lower volumes of,