New pyrazoline-basedpHfluorescentpr

New pyrazoline-basedpHfluorescentprobeshavebeensynthesizedandcharacterized.Theseprobes
respond toacidicpHwithshortresponsetime(lessthan1min),highselectivityandsensitivity.They
have goodreversibilityandnearlynointerferencefromcommonmetalions.Theseprobesaresuitablefor
strong acidconditionsandcanquantitativelydetectpHvaluebasedonequilibriumequation,pH = pKa −
log(Ia − Ix)/(Ix − Ib)

, withverygoodlinearrelationship.Moreover,using 1H NMRanalysis,weverified
that theprotonationofthenitrogeninthiazoleweakenedthethiazolering’sabilitytodonateelectrons
to pyrazolinemoietyandchangedtheintramolecularchargetransferthatwasactuallythemechanism
of thefluorescencequenchingphenomenonunderstronglyacidicconditions.Moreimportantly,these
probes canimagestrongacidityin Saccharomyces cerevisiae.

ผลลัพธ์ (ไทย) 2:

pyrazoline-basedpHfluorescentprobeshavebeensynthesizedandcharacterized.Theseprobes
ใหม่ตอบสนองtoacidicpHwithshortresponsetime (lessthan1min) highselectivityandsensitivity.They
มี goodreversibilityandnearlynointerferencefromcommonmetalions.Theseprobesaresuitablefor
acidconditionsandcanquantitativelydetectpHvaluebasedonequilibriumequation แข็งแกร่ง pH = pKa? -
เข้าสู่ระบบ (เอีย - Ix) / (ทรงเครื่อง -
Ib)?,
withverygoodlinearrelationship.Moreover ใช้ 1H NMRanalysis, weverified
ที่ theprotonationofthenitrogeninthiazoleweakenedthethiazolering'sabilitytodonateelectrons
เพื่อ pyrazolinemoietyandchangedtheintramolecularchargetransferthatwasactuallythemechanism
ของ thefluorescencequenchingphenomenonunderstronglyacidicconditions.Moreimportantly เหล่านี้ยานสำรวจ canimagestrongacidityin Saccharomyces cerevisiae

ผลลัพธ์ (ไทย) 3:

ใหม่ pyrazoline basedphfluorescentprobeshavebeensynthesizedandcharacterized . theseprobes
ตอบ toacidicphwithshortresponsetime ( lessthan1min ) highselectivityandsensitivity พวกเขามี goodreversibilityandnearlynointerferencefromcommonmetalions

. theseprobesaresuitablefor  แข็งแรง acidconditionsandcanquantitativelydetectphvaluebasedonequilibriumequation pH = pKa −
ล็อก ( IA − 9 ) ( 9  − IB )

,withverygoodlinearrelationship นอกจากนี้ การใช้ nmranalysis weverified
ที่ 1 , theprotonationofthenitrogeninthiazoleweakenedthethiazolering'sabilitytodonateelectrons

เพื่อ pyrazolinemoietyandchangedtheintramolecularchargetransferthatwasactuallythemechanism ของ thefluorescencequenchingphenomenonunderstronglyacidicconditions . moreimportantly เหล่านี้
) canimagestrongacidityin Saccharomyces cerevisiae .