) and maintained on
potato dextrose agar medium containing 200 g extract of boiled
potatoes, 20 g of glucose, and 20 g of agar in 1 L of distilled water at
4 C. Conidial suspensions of the pathogens were prepared by
flooding for 5 d the old cultures incubated on plates at 28 C with
sterile distilled water containing 0.1 g kg1 Tween-80. Spore concentrations
were determined with a hemocytometer and adjusted
as required with sterile distilled water.