To further characterize cell adhesion to materials surface a SEM analysis was also performed (see Fig. 3A). Fibroblasts attached and spread across the surface, presenting a round shape configuration, with some cytoplasm extensions toward the substrate after 24 h of being seeded on CAH surface. After 72 h, cells already presented the typical fibroblastic morphology, lamellipodia connecting to surrounding fibroblast were visualized and a continuous layer of cells started to be formed . The cell behavior observed can be explained by the interaction of glycosaminoglycans, present at cell membrane, with the amine groups of chitosan present in CAH ). Moreover, chitosan provides a matrix for 3D tissue growth and activates macrophages at the wound site that stimulate cell proliferation and histoarchitectural tissue organization