Following heat-induced antigen retrieval,
immunohistochemical staining was performed on wax sections
with rabbit polyclonal anti-Glycogen Phosphorylase H-300 (sc-
66913; Santa Cruz Biotechnology, Dallas, TX, USA) and anti–
phospho-Glycogen-Synthase (07-817; Merck Millipore, Temecula,
CA, USA).