The test mixtures were notated according to the detected level
of BD, e.g. “24%”. They were produced by incubation of the below
components with 3% (m/v) of Lypozyme TL HC at 35 ◦C, 200rpm
(all compounds in relative volumes v): (1) 0.92 v oil, 0.04 v water
for quantitative analysis. The relevant calibrations are presented, and the general principles of analysis of
nonlinear responses are discussed. Several experimental samples were produced by enzymatic conversion
of rapeseed oil to BD. One reaction step resulted in 85–95% conversion (6 h). The second step (after
removal of glycerol and water) increased the yield to 97–98%. All components of the mixtures were separated
and quantified. Relation of the BD contents measured by TLC and GC gave the values of 1.03±0.07
(TLC-staining) and 0.95±0.04 (TLC–FID), indicating applicability of the TLC-methods