Steaming was carried out using a steam cooker (Braun GmbH, Frankfurter Straße, Kronberg, Germany) and with a single layer of the fresh cauliflower (300 g) samples suspended above 400 mL of boiling water for 10 min. Boiling was carried out with 1 L of water for 10 min. The fresh cauliflower florets (300 g) samples were added to already boiling water (98 °C). Samples were immediately cooled and lyophilized with Hetosicc GF-765 (Heto, Birkerød, Denmark). The freeze-dried samples were ground to a fine powder using a mill and stored at −20 °C until analyses.
2.4. Moisture analysis