Homogenized egg samples (100 ml) were artificially
inoculated with 100 ul suspensions of SE cells
to make final a concentration of approximately 10
cells/ml. The spiked samples were mixed with buffered
peptone water (BPW) giving egg to broth ratios
of 1:2, 1:4 and 1:10, and further stomached as
described before. After 24 h incubation at 37 jC,
100 ul of each broth culture treatment was applied
onto the test panel and the results were determined
after 20 min.