Microbiological analyses were performed by surface spread plating
with each sample diluted in peptone water (0.1%). Total mesophilic
aerobic bacteria were counted on standard Plate Count Agar (PCA)
incubated at 35oC for 48 hours and expressed in colony forming units
per gram (CFU g-1). The count of filamentous fungi and yeast was
performed on Potato Dextrose Agar medium (PDA) acidified with
10% tartaric acid. The plates were incubated at 25oC for 3-5 days and
the results expressed in CFU/g.