Effect of temperature
Temperature affects enzyme activity in much the same way
as it affects other chemical reactions. Rates increase by
between 4 and 8% per degree C, although at high
temperatures denaturation of the enzyme protein decreases
product formation. Thus it is important when
carrying out an enzyme assay to ensure that the temperature
remains constant, and also that you know exactly
what it is. For comparison with other results that may have
been reported at other temperatures, the exact temperature
coefficient should be known; if not, a figure of 6% per
degree is a useful approximation.