In order to study the effect of TC on maltose fermentation at5◦C, maltose and glucose media of various concentrations were fermented using the TC/S. cerevisiae AXAZ-1 biocatalyst at this temperature. Fermentations were also carried out using free yeast cells in suspension. Fig. 1 shows that the free yeast cells (FC) were not able to ferment maltose (100 g/l) at 5◦C in contrast to glucose,which was completely fermented. On the other hand, in Fig. 2 it is shown that the use of immobilized cells sharply accelerated the rate of maltose fermentation compared to FC. Therefore, immobilization on TC seems to increase the yeast efficiency for maltose trans-port, hydrolysis and fermentation. All maltose media (80, 100 and140 g/l) were completely fermented, in times ascending at higher maltose concentrations, but clearly indicating a positive effect of yeast immobilization on maltase activity. Kinetic parameters (fermentation time, residual sugar and ethanol concentration) of the fermentations of the different maltose and glucose media using immobilized cells at 5◦C are presented in Table 1. The sugar conversion percentages of maltose and glucose were similar (above 93%)but higher yields of ethanol were obtained in the case of maltose fermentations.