3. Results
3.1. Tolerance of pathogens to hot water
Water temperature and dipping time considerably influenced
mycelium development of pathogens (Table 1). L. theobromae was
more sensitive to higher temperature and longer exposure than
C. gloeosporioides. Mycelium growths of both pathogens were
partially inhibited by HWT regardless of trial, temperature and
dipping time. The maximum reduction in radial growth of
C. gloeosporioides after 7 days was 79.33% at 52 C, 50 min whilst
the highest decrease in mycelium development of L. theobromae
was 90.5% at 53 C, 50 min. Reduction in spore germination of
C. gloeosporioides after HWT is summarized in Table 2. Similarly,
spore germination was not totally inhibited even with the highest
temperature and exposure tested. Nevertheless spore germination
significantly decreased as temperature increased.
Analysis of variance (ANOVA) showed that temperature significantly
(P ¼ 0.05) influenced the radial growth of C. gloeosporioides
while trial and exposure had insignificant effect. The interaction
effect of trial, temperature and exposure on colony diameter was
insignificant, too. Meanwhile, temperature and exposure or their
interactions considerably influenced the mycelium growth of
L. theobromae. The interaction effect of trial, temperature and
exposure was not significant on colony development of
L. theobromae. Meanwhile, temperature and exposure or their
combinations had significant effect on spore germination of
C. gloeosporioides.
3.2. Tolerance of fruit to hot water
HWT at 48e50 C, 10e50 min and 52 C, 10e20 min was not
injurious to ‘Carabao’ mangoes after 14 days. Fruit dipped at 52 C
for 30e50 min had no injury immediately after treatment but LLBS