Methods
Sample collection
This study included 86 field archival isolates of C. suis
from years 2011–2013, which were derived from clinically
healthy animals and pigs with clinical disorders (i.e.
conjunctivitis cases and reproductive disorders in sows).
The research was conducted in closed-loop farms with
20–120 adult sows in each basic herd. Ten farms were
tested. The required sample size was computed in
WinEpiscope 2.0 (EPIDECON), assuming an expected
prevalence of 30% and a confidence level of 95%.
Conjunctival swabs from both eyes and a swab from
the vaginal vestibule were collected from each sow. The
specimens were stabilized by using the PAXgene Tissue