3. DNA analysis
3.1. Specimens
Flies were collected from human corpses (see cases 1–3), by net
or by canopy trap. Mixed bait of beef liver and dimethyl trisulphide
was used. Morphological identification was conducted based on
Rognes [5]. The genitalia of the males (16 of 23 in total) were
adjusted with the line-drawings of Rognes [5] supplementary to
the characteristics provided by the original key. Afterwards, the
specimens were stored in 95% EtOH. Specimens identified for this
study are listed in Table 1. Two legs were taken from each
specimen and DNA-extraction was conducted according to a usual
phenol–chloroform-protocol. The purified DNA was resuspended
in 100 ml of sterile distilled water.