Abstract Cotton leaf curl virus (CLCuV) is a serious pathogen causing leaf curl disease and affecting
the cotton production in major growing areas. The transgenic cotton (Gossypium hirsutum cv.
Coker 310) plants were developed by using bC1 gene in antisense orientation gene driven by
Cauliflower mosaic virus-35S promoter and nos (nopaline synthase) terminator and mediated by
Agrobacterium tumefaciens transformation and somatic embryogenesis system. Molecular confirmation
of the transformants was carried out by polymerase chain reaction (PCR) and Southern blot
hybridization. The developed transgenic and inoculated plants remained symptomless till their
growth period. In conclusion, the plants were observed as resistant to CLCuV.
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