Samples consisted of unfixated neat semen, neat semen fixated 1:6 (v/v) in 1% PBS-buffered formalin, and semen diluted with commercial BTS extender (MinitübGmbH, Germany). Samples were transported by commercial courier, using temperature-controlled packaging in styrofoam boxes. Upon arrival in the laboratory, the temperature of the samples was measured using a Flash III infrared thermometer (Hartenstein, Germany). Neatand fixated samples were examined immediately uponarrival. Extended semen was stored under light-protectedconditions at 16–18◦C in climate-controlled chambers (Kli-maKit, Minitüb GmbH, Germany) and then was analyzed the following day by two experienced laboratory specialists.