transformation into plantlets.
For isolated microspore culture, flower buds were
selected as described for anther culture. Their locular
content was isolated by filtration, plated and incubated
at 35C for 3 days according to (Miyoshi 1996), and
then continuously at 25C in darkness, suspended in
liquid NLN culture medium (Lichter 1982) supplemented
with 2% sucrose, 0.5 mg/l naphthaleneacetic
acid (NAA), and 0.5 mg/l 6-benzylaminopurine
(BAP), pH 5.9, for cell proliferation and production
of embryo-like structures.