2.8. Data analysis and optimization
Statistical software SPSS (version 16) was used to fit the response surface model to the experimental data through the
multiple regression analysis. Optimization of the culture conditions in terms of glucose, peptone and tween 80 was analyzed using the predictive polynomial model through solver function. Using Statistic V software, three dimensional plots were created by holding two variables of the model as constants. All data are the mean of triplicates unless it is stated otherwise
and their ±SD were almost negligible.