Preparation of plant extracts. Mature fruits were collected and dried in an oven under circulating air at 45˚C for weight stabilization. The fruits were then ground in a knife mill. The extract was obtained by turbo extraction of 50 g of the sample in 500 mL 70% (w/w) alcohol for 15 minutes followed by filtration in a rotary evaporator under reduced pressure at a maximum temperature of 40˚C until complete elimination of the organic solvent. The turbo extraction technique does not allow the temperature to exceed 40˚C. This procedure was performed because it was considered relatively harmless regarding the extraction of different components. The sample was then lyophilized until all water was removed. For the physicochemical quality control tests, 10 g of mature fruit was dried at room temperature in the dark - See more at: http://www.woundsresearch.com/article/effect-glycolic-extract-dillenia-indica-l-combined-microcurrent-stimulation-experimental-les#sthash.Bos504cs.dpuf