More importantly, these experiments
indicate that the degradation of the Griess reagent during the
manufacturing and storage process could be minimized by simply
spotting the sulfanilamide and NED in separate wells and limiting
their exposure to oxygen.
This could be efficiently achieved by placing
the devices under nitrogen in a disposable plastic bag. Although
the requirement of nitrogen to dry the PADs (after introducing
the sample) could hinder the development of some point-of-care
applications involving the analysis of low concentrations of nitrite,
it is envisioned that professionals can still use the PADs at on-site
diagnosis centers.