In order to select lipase-producing fungal strains, their ability tosecrete extracellular lipases was tested by the detection of halos in a nutrient medium containing rhodamine (8.0 mL L−1) as described by Castro-Ochoa et al. prepared with (g L−1) 0.01 rhodamine B (Sigma–Aldrich); 4.0 NaCl, 10.0 agar, and 30.0 soybean oil. Lipase activity was detected as a fluorescent halo under UV light around the colonies after 24 h of incubation, measuring its diameter with a ruler graduated in centimeters.