2.7. Relative electrolyte leakage (REL)
REL was determined in sprayed leaves of two weed species (E.crus-galli and C. occidentalis) to study the effect of Artemisia oil on solute leakage and thus membrane integrity as per Singh et al. (2008). Leaf tissue (100 mg) was immersed in distilled water (bathing medium) for 30 min and the conductivity of medium was measured (C1). Thereafter, test tubes containing leaf tissues were boiled for 15 min and the conductivity was again measured (C2).The relative electrolyte leakage (REL) was calculated using following formula and expressed in percent.