TS content was determined, in duplicate, according to Gon ̃i,Garcı ́a-Alonso, and Saura-Calixto (1997). Samples (50 mg) were suspended in 2 mol L1KOH to disperse starch and shaken at room temperature for 30 min. The samples were incubated (60 C,45 min, pH 4.75) with amyloglucosidase (1 mL (300 Um L 1), SigmaA-7255) to hydrolyze starch. The free glucose was determined using glucose oxidase, peroxidase and ABTS assay (Bergmeyer andBernet, 1974). The total starch content was calculated as glucose คูณ0.9. The wheat starch (Sigma S-1514) was used as a reference standard.