Rooting Stage
Macroscopic and microscopic identification was
performed when the length of the roots of the sprouts
reached a level of 10-20 mm. The developmental stages
of the soybean seed is given in Figure 4. All tissue
culture studies were performed with three replicates.
Plant growth regulators, such as brassinosteroids,
accelerate efficient seed germination and vegetative
growth against environmental stress factors during the
growth and development of flwering plants, including
soybean [15]. In the present study, for the acceleration of
seed germination, the MS medium (1-3mg/l) was added
indole acetic acid (IAA) and gibberellic acid (GA3) [9].
Germination trials were controlled. The root, stem and
leaf explants of the plant formed within 45 days, followed
by the conduct of pot and field trials. Accordingly, it was