The sugars were determined from 0.02 mL of the extract used for amino acid analysis. Glucose, fructose and sucrose contents were analysed by ion chromatography (Metrohm 850 system) using injection valve along with the Pixel Array Detector (PAD) anion exchange column (1–150 Metrosep-Carb) and isocratic highpressure pump for the channel PAD 818 IC.