A previous study showed that heat-activated
spores of a food poisoning C. perfringens isolate (strain SM101)
can germinate at temperatures lethal to vegetative cells (i.e., higher
than 55 C) when incubated in 25 mM phosphate buffer (pH 7.0)
with 100 mM L-asparagine (AK mixture) and 100 mM KCl, but not
when incubated with KCl or L-asparagine alone (Paredes-Sabja et al.,
2008d).