In
this
work,
hemp
(Cannabis
sativa
L.)
seed
oil
was
produced
by
extraction
with
supercritical
CO2 under
different
extraction
conditions
(temperature,
pressure
and
time).
The
objective
was
to
evaluate
the
influ
ence
of
extraction
conditions
on
concentration
of
tocopherols,
fatty
acids
and
pigments
in
hemp
seed
oil.
The
composition
of
hemp
seed
oil
obtained
with
supercritical
CO2 was
compared
with
the
hemp
oil
extracted
by
n-hexane
using
Soxhlet
method
and
with
oil
obtained
by
pressing
using
screw
expeller.
Using
supercritical
CO2 extraction
the
extracts
higher
in
concentration
of
tocopherol
were
produced.
The
amount
of
tocopherol
in
supercritical
extracts
ranged
from
37.09
to
110.61
mg
L−1,
depending
on
the
applied
process
conditions,
while
-tocopherol
content
was
significantly
higher
(2–3
times).
The
content
of
pigments
in
the
hemp
oil
obtained
by
supercritical
CO2 had
been
changed
significantly
during
the
extraction
time
from
9.79
to
178.76
mg
kg−1 for
total
chlorophyll
content
and
8.15
to
57.66
mg
kg−1
for
total
carotene
content.
By
selecting
the
relevant
process
conditions
of
supercritical
extraction
it
is
possible
to
obtain
hemp
seed
oil
with
physical
or
nutritional
properties
of
interest
to
the
food
industry.