Data were summarized using means, medians, and
standard deviation and evaluated for normality using
the Kolmogorov-Smirnov test and for equal variance
with Levene’s test. Comparisons of BCI with visual
BC categories, TS values, and biochemical values
were done using ANOVA with post hoc Tukey
pairwise comparisons for data meeting assumptions
of equal variance and normality. Non-parametric
Kruskal-Wallis ANOVA and post hoc Bonferroni
pairwise testing was done for data not meeting
assumptions of normality and equal variance. Spearman
correlations were tested between BCI and biochemical
values. Stepwise multiple linear regression
was conducted to evaluate the influence of biochemical
parameters of healthy and FP-afflicted turtles on
BCI. Reference intervals for healthy animals were
expressed as mean ± 2SD or 2.5−97.5th percentiles
for data that were not normally distributed (LabradaMartagón
et al. 2013). The level of significance for all
statistical tests was α = 0.05, and all statistics tests
were performed using SPSS 20.0.