Serial dilutions of samples were prepared
by washing vigorously 10 g tissue with 90 mL of 1 g/L sterile
buffered peptone water in sterilized round bottom tubes at room
temperature and inoculated over plate count agar (PCA) at 35 C for
48 h and potato dextrose agar (PDA) supplemented with 0.05 g/L
chloramphenicol at 21 C for 5e7 days, respectively by the spread
plate method (ICMSF, 1978).