In conclusion, we established suitable media with different composition of phytohormones for Micro propagation of Banana cv. Agnishwar: (1) for multiple shoot regeneration directly from shoot tip, the suitable medium was MS + 4.0 mg/l BAP, (2) for shoot elongation, the suitable medium was MS + 5.0 mg/l BAP and (3) for rooting ½ MS + 1.0mg/l IBA. Results of this study indicate that the cultures showing higher rate of multiplication in the first two or three subcultures continued this behavior in the next sub-culturing. Therefore, from the commercial point of view, these media composition might be applied for large scale propagation of healthy and disease free banana cv. Agnishwar plan