SCSF was prepared by centrifuging one set of 1-5 SS for 15 min at 5000 rpm. The
supernatant was separated gently and were passed through millipore membrane filter (Sartorius,
pore dia. 0.45 µm, 25 mm circles) to eliminate all germs from the suspensions. A second set of
similar supernatant from SCSF was autoclaved at 121ºC for 15 minutes and marked as ASS. All
the experiments regarding SS 1-5, SCSF and ASS were carried out with dry seeds of BR-26.
There were three replicates in each set and a control containing the same amount of sterile distilled
water instead of SS.