The aim of these experiments was to identify an ethanol con-centration having a clear in fl uence in the growth kinetics of the
original yeast strains (i.e. constituting a selective pressure), while
still maintaining a dilution rate that would allow to get hundreds of
generations in just a few weeks. The strains were cultivated in YPD5
medium supplemented with different amounts of ethanol, and
growth kinetics was monitored as described in Materials and
Methods. Both strains were able to grow in the presence of each