Screening of amylolytic property of the strains was based on starch hydrolysis method. Initially, 07 fungal strains were selected and among them 05 filamentous fungi including A. fumigatus KIBGE-IB33, A. flavus KIBGE-IB34, A. terreus KIBGE-IB35, A. niger KIBGE-IB36 and A. versicolor KIBGE-IB37showed production for amylolytic enzymes. When these isolates were cultivated in the starch containing production medium, highest titers of α-amylase (11.0 kUmg-1) and amyloglucosidase (142.0 kUmg-1) were produced by A. fumigatus KIBGE-IB33 (Figure 1). This strain was also capable of producing considerable amount of glucose (81.0 gL-1) which can be used for the production of ethanol. The fermentable sugar produced by this isolate can be easily metabolized by S. cerevisiae. However, other filamentous fungi produced lower titers of both α-amylase and amyloglucosidase along with lower concentration of glucose. Although maximum α-amylase was produced by A. flavus KIBGE-IB34 but it did not showed higher glucose formation rate as compared to A. fumigatus KIBGE-IB33 therefore, this isolate was selected for further studies.