The DPPH radical scavenging ability of the ten lotus extracts was determined according to the method previously described by Wang et al. (2008) with slight modifications. In brief, individual methanol diluted lotus extract (ranged from 6.25 to 100 g/ml) was added to 2 ml of methanol containing DPPH radical (0.1 mM) followed by vigorous shaking. After standing in darkness for 30 min, absorbance of the mixture was measured colorimetrically at 517 nm. The DPPH radical scavenging ability of individual lotus extract was calculated by using following equation: