In this paper we aim to demonstrate

In this paper we aim to demonstrate,asaproof-of-concept,thefeasibilityofthemassproductionof
effectivepointofcaretestsfornitritequantification inenvironmental,foodandclinicalsamples.Fol-
lowing ourpreviousworkonthedevelopmentofthirdgenerationelectrochemicalbiosensorsbasedon
the ammoniaforming nitrite reductase (ccNiR), hereinwereducedthesizeoftheelectrodes'systemtoa
miniaturized format,solvedtheproblemofoxygeninterferenceandperformedsimplequantification
assaysinrealsamples.Inparticular,carbonpastescreenprintedelectrodes(SPE)werecoatedwitha
ccNiR/carbon inkcompositehomogenizedinorganicsolventsandcuredatlowtemperatures.Thebio-
compatibility ofthesechemicalandthermaltreatmentswasevaluatedbycyclicvoltammetryshowing
that thecatalyticperformancewashigherwiththecombinationacetoneanda40 °C curingtemperature.
The successfulincorporationoftheproteininthecarbonink/solventcomposite,whileremainingcata-
lyticallycompetent,attestsforccNiR's robustnessandsuitabilityforapplicationinscreenprintedbased
biosensors.
Becausethedirectelectrochemicalreductionofmolecularoxygenoccurswhenelectroanalyticalmea-
surementsareperformedatthenegativepotentialsrequiredtoactivateccNiR (ca.
0.4 VvsAg/AgCl),an
oxygenscavengingsystembasedonthecouplingofglucoseoxidaseandcatalaseactivitieswassuccessfully
used. Thisenabledthequantificationofnitriteindifferentsamples(milk,water,plasmaandurine)ina
straightforwardwayandwithsmallerror(1–6%).Thesensitivityofthebiosensortowardsnitritereduction
under optimizedconditionswas0.55AM
1 cm
2 withalinearresponserange0.7–370 μM.

ผลลัพธ์ (ไทย) 1:

ในเอกสารนี้ เรามุ่งมั่นที่แสดงให้เห็นถึง asaproof-ของแนวคิด thefeasibilityofthemassproductionofeffectivepointofcaretestsfornitritequantification inenvironmental, foodandclinicalsamples Fol-ourpreviousworkonthedevelopmentofthirdgenerationelectrochemicalbiosensorsbasedon ควายเหล็กammoniaforming ไนไตรต์ reductase (ccNiR) hereinwereducedthesizeoftheelectrodes'systemtoaรูป miniaturized, solvedtheproblemofoxygeninterferenceandperformedsimplequantificationassaysinrealsamples Inparticular, carbonpastescreenprintedelectrodes (SPE) werecoatedwithainkcompositehomogenizedinorganicsolventsandcuredatlowtemperatures ccNiR/คาร์บอน Thebio-ofthesechemicalandthermaltreatmentswasevaluatedbycyclicvoltammetryshowing กันcuringtemperature ° C ที่ thecatalyticperformancewashigherwiththecombinationacetoneanda40Whileremainingcata successfulincorporationoftheproteininthecarbonink/solventcomposite การ-lyticallycompetent, robustnessandsuitabilityforapplicationinscreenprintedbased ของ attestsforccNiRbiosensorsBecausethedirectelectrochemicalreductionofmolecularoxygenoccurswhenelectroanalyticalmea-surementsareperformedatthenegativepotentialsrequiredtoactivateccNiR (ca. 0.4 VvsAg/AgCl), การoxygenscavengingsystembasedonthecouplingofglucoseoxidaseandcatalaseactivitieswassuccessfullyใช้ อิ Thisenabledthequantificationofnitriteindifferentsamples (นม น้ำ plasmaandurine)straightforwardwayandwithsmallerror(1–6%) Thesensitivityofthebiosensortowardsnitritereductionภายใต้ optimizedconditionswas0.55AM ซม. 1 2 μM withalinearresponserange0.7-370

ผลลัพธ์ (ไทย) 2:

ในบทความนี้เรามุ่งมั่นที่จะ
inenvironmental, foodandclinicalsamples.Fol-
ควายเหล็ก ourpreviousworkonthedevelopmentofthirdgenerationelectrochemicalbiosensorsbasedon
reductase ไนไตรท์ ammoniaforming (ccNiR) hereinwereducedthesizeoftheelectrodes'systemtoa
ขนาดเล็ก inkcompositehomogenizedinorganicsolventsandcuredatlowtemperatures.Thebio- เข้ากันได้ ofthesechemicalandthermaltreatmentswasevaluatedbycyclicvoltammetryshowing thecatalyticperformancewashigherwiththecombinationacetoneanda40 ว่า° C curingtemperature. (แคลิฟอร์เนีย? 0.4 optimizedconditionswas0.55AM 1 ซม. 2 withalinearresponserange0.7-370 ไมครอน

ผลลัพธ์ (ไทย) 3:

ในกระดาษนี้เรามุ่งหวังที่จะแสดงให้เห็นถึง , asaproof แนวคิด thefeasibilityofthemassproductionof
effectivepointofcaretestsfornitritequantification สิ่งแวดล้อม foodandclinicalsamples fol -
, .
ourpreviousworkonthedevelopmentofthirdgenerationelectrochemicalbiosensorsbasedon lowing ammoniaforming ไนไตรต์รีดักเตส ( ccnir ) hereinwereducedthesizeoftheelectrodes'systemtoa
ขนาดเล็กรูปแบบsolvedtheproblemofoxygeninterferenceandperformedsimplequantification
assaysinrealsamples โดยที่ carbonpastescreenprintedelectrodes ( SPE ) werecoatedwitha
ccnir / คาร์บอน inkcompositehomogenizedinorganicsolventsandcuredatlowtemperatures . thebio ofthesechemicalandthermaltreatmentswasevaluatedbycyclicvoltammetryshowing
-
เข้ากันได้ที่ thecatalyticperformancewashigherwiththecombinationacetoneanda40 ° C curingtemperature .
-
successfulincorporationoftheproteininthecarbonink / solventcomposite whileremainingcata , lyticallycompetent attestsforccnir , ของ robustnessandsuitabilityforapplicationinscreenprintedbased
-

becausethedirectelectrochemicalreductionofmolecularoxygenoccurswhenelectroanalyticalmea ตาม .surementsareperformedatthenegativepotentialsrequiredtoactivateccnir ( ประมาณ
0.4 vvsag 0.46% ) , oxygenscavengingsystembasedonthecouplingofglucoseoxidaseandcatalaseactivitieswassuccessfully

ใช้ . thisenabledthequantificationofnitriteindifferentsamples ( นม , น้ำ , plasmaandurine ) (
straightforwardwayandwithsmallerror ( 1 ) thesensitivityofthebiosensortowardsnitritereduction
6 % )ภายใต้ optimizedconditionswas0.55am
1 ซม.
2 withalinearresponserange0.7 μ– 370 เมตร