The germination rate and percentage of seeds treated with 0.25 μM colchicine tended to be higher than that of the 25 μM treatment (p b 0.05), although the difference was not statistically significant (Fig 1). However, colchicine had a significant negative effect on germination for both the 0.25 μM overnight and 25 μM three day treatments,when compared with the control. The differences in germination rate manifested five to 6 weeks after initial exposure to colchicine.Similar findings have been reported on a wide range of species (Ramsey and Schmeske, 1998).