Reference strain of A. hydrophila 19570 was obtained
from American Type Culture Collection (ATCC), USA.
Bacteria was grown in tryptic soy agar (TSA) plates and
broths (TSB) at 22-25oC for 24 to 48 h. Stock cultures were
maintained in a broth medium supplemented with glycerol
at -20oC (Austin and Austin, 1999).