and spots corresponding
to TAG and themajor phospholipids were scraped, spikedwith a known
standard (15:0), and transesterified for GC/MS analysis to determine
the fatty acid composition as well as to determine the relative levels of
TAG, phosphatidylethanolamine (PE), and phosphatidylcholine (PC)
fractions. At least three biological replicates were used for TLC analysis.
Significance was determined by t-test calculation using GraphPad Prism
5 software.