3.3. Effects of fraction H2 on cell surface expression of CD4, CD8 and CD14 in PBMC
To evaluate the effects of fraction H2 on cell surface expression of CD4, CD8 and CD14 in PBMC, cells were treated with a series of concentrations of fraction H2 from 200 to 800 μg/ml for 72 h. In Fig. 5A, fraction H2 at concentration of 800 μg/ml induced significant increase in CD14+ lymphocyte population in total lymphocytes. However, the CD4+/CD8+ ratio was not changed by fraction H2 treatment (Fig. 5B).