The unhopped lager base beer used for this study was prepared atthe 10 hL SABMiller research brewery at the Sutton Bonington campusof the University of Nottingham. The standard brew (5% ABV) was prepared from a grist composition of 70% pilsner malt and 30% dextrose adjunct. Mash-in temperature was 48 °C with addition of CaCl2 at a rate of100 mg/L. This was followed by wort boiling for 60 min (5% evaporation) and a 15 min trub stand time. The wort was cooled and fermentedwith a standard SABMiller lager yeast for 10 days, and maturationfollowed for 4 days. The beers were packaged in 330 mL brown bottlesand stored at 3 °C until their preparation for sensory appraisal. The original gravity and pH of the beer were 1.044 and 4.23, respectively