Conclusions
The SSELP process was superior to other processes in terms of converting alkaline-pretreated
corn stover into lipids by C. curvatus, as it required less cellulolytic enzymes and had a
higher lipid coefficient. Moreover, the process facilitated easy enzyme recycling that should
lead to further reduction of enzyme consumption. These results provide valuable information
for cost-effective lipid production from lignocelluloses, which should be particularly
important in achieving a sustainable production of biodiesel.