Strain CS-1 was pregrown on BSGYP medium for 24 h. Erlenmeyer
flasks containing 100 mL of growth medium (pH 8) and
3 g of milled rice straw was inoculated with 2 mL of pregrown pure
culture (0.65 mg protein mL1). Uninoculated (control) and bacterial
inoculated flasks were incubated at 37 C on a rotary shaker
(120 rpm) in dark conditions for 3 days.
Rice straw was provided by a farmhouse which cultivates Korean
rice in Icheon, Gyeonggi, Korea. Air dried raw material without
classification were cut into 3–5 cm lengths and stored in sealed
plastic bags at room temperature for pretreatment. Prior to composition
analysis, biomass was ground using a Wiley mill and, particles
between the sizes of 40 and 80 mesh were collected. Milled
rice straws were washed with water to remove dust and dried in
an air forced oven at 60 C for 48 h.
As a control, Thermobifida fusca (NBRC 14071T), obtained from
NITE Biological Resource Center in Japan was used for the removal
of lignin in rice straw. Cultivation was performed with nutrient
broth medium (pH 6.0) and incubated at 45 C on a rotary shaker
(170 rpm) in dark conditions for 10 days.
Strain CS-1 was pregrown on BSGYP medium for 24 h. Erlenmeyer
flasks containing 100 mL of growth medium (pH 8) and
3 g of milled rice straw was inoculated with 2 mL of pregrown pure
culture (0.65 mg protein mL1). Uninoculated (control) and bacterial
inoculated flasks were incubated at 37 C on a rotary shaker
(120 rpm) in dark conditions for 3 days.
Rice straw was provided by a farmhouse which cultivates Korean
rice in Icheon, Gyeonggi, Korea. Air dried raw material without
classification were cut into 3–5 cm lengths and stored in sealed
plastic bags at room temperature for pretreatment. Prior to composition
analysis, biomass was ground using a Wiley mill and, particles
between the sizes of 40 and 80 mesh were collected. Milled
rice straws were washed with water to remove dust and dried in
an air forced oven at 60 C for 48 h.
As a control, Thermobifida fusca (NBRC 14071T), obtained from
NITE Biological Resource Center in Japan was used for the removal
of lignin in rice straw. Cultivation was performed with nutrient
broth medium (pH 6.0) and incubated at 45 C on a rotary shaker
(170 rpm) in dark conditions for 10 days.
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