. In this study, we developed
SERS-based assays targeting the hPSC surface markers stagespecific
embryonic antigen-5 (SSEA-5) and TRA-1-60 to detect residual
undifferentiated hPSCs with high specificity and sensitivity.
Using our newly developed assays, we efficiently detected SSEA-5þ
and TRA-1-60þ cells at sensitivities several orders of magnitude
higher than flow cytometry assays.